Small-angle X-ray scattering probe of intermolecularinteraction in red blood cells

LIU Guan-Fen; WANG We-Jia; XU Jia-Hua; DONG Yu-Hui

doi:10.1088/1674-1137/39/3/038001

Chinese Physics C> 2015, Vol. 39> Issue(3) : 038001 DOI: 10.1088/1674-1137/39/3/038001

Small-angle X-ray scattering probe of intermolecularinteraction in red blood cells

Abstract
HTML
Reference
Related

PDF

Abstract：
With high concentrations of hemoglobin (Hb) in red blood cells, self-interactions among these molecules could increase the propensities of their polymerization and aggregation. In the present work, high concentration Hb in solution and red blood cells were analyzed by small-angle X-ray scattering. Calculation of the effective structure factor indicates that the interaction of Hb molecules is the same when they are crowded together in both the cell and physiological saline. The Hb molecules stay individual without the formation of aggregates and clusters in cells.

[1]	A.B. Arbuzov , S.G. Bondarenko , I.R. Boyko , L.V. Kalinovskaya , A.A. Kampf , R.R. Sadykov , V.L. Yermolchyk . ${\mathrm{SANC}}$ Monte Carlo programs for small-angle Bhabha scattering. Chinese Physics C, 2024, 48(4): 043002. doi: 10.1088/1674-1137/ad1fe6
[2]	Wen-Qiang Hua , Yu-Zhu Wang , Ping Zhou , Tao Hu , Xiu-Hong Li , Feng-Gang Bian , Jie Wang . Microfocus small-angle X-ray scattering at SSRF BL16B1. Chinese Physics C, 2017, 41(4): 048001. doi: 10.1088/1674-1137/41/4/048001
[3]	Tai-Sen Zuo , He Cheng , Yuan-Bo Chen , Fang-Wei Wang . Development and prospects of Very Small Angle Neutron Scattering (VSANS) techniques. Chinese Physics C, 2016, 40(7): 076204. doi: 10.1088/1674-1137/40/7/076204
[4]	ZHAO Bo-Zhen , QIN Xiu-Bo , FENG Zhao-Dong , WEI Cun-Feng , CHEN Yan , WANG Bao-Yi , WEI Long . Performance evaluation of CsI screens for X-ray imaging. Chinese Physics C, 2014, 38(11): 116003. doi: 10.1088/1674-1137/38/11/116003
[5]	DING Yun-Ze , DU Ying-Chao , ZHANG Zhen , HUANG Wen-Hui . Simulation study of a photo-injector for brightness improvement in Thomson scattering X-ray source via ballistic bunching. Chinese Physics C, 2014, 38(2): 027003. doi: 10.1088/1674-1137/38/2/027003
[6]	WANG Shuo , JI Jian-Feng , HAN Dong , FENG Hua . Simulation of photoelectric X-ray polarimetry and reconstruction of the photoelectron azimuthal angle. Chinese Physics C, 2013, 37(3): 036002. doi: 10.1088/1674-1137/37/3/036002
[7]	ZHANG Fei , WANG Huan-Yu , PENG Wen-Xi , LIANG Xiao-Hua , ZHANG Chun-Lei , CAO Xue-Lei , JIANG Wei-Chun , ZHANG Jia-Yu , CUI Xing-Zhu . High resolution solar soft X-ray spectrometer. Chinese Physics C, 2012, 36(2): 146-150. doi: 10.1088/1674-1137/36/2/008
[8]	DU Ying-Chao , HUA Jian-Fei , YAN Li-Xin , DU Qiang , HUANG Wen-Hui , TANG Chuan-Xiang . Bunch length measurement at Tsinghua Thomson scattering X-ray source. Chinese Physics C, 2011, 35(5): 505-508. doi: 10.1088/1674-1137/35/5/020
[9]	WANG De-Hong , HAO Jun-Jie , XING Xue-Qing , MO Guang , GONG Yu , WU Zhong-Hua , . Characterization of the nanopore structures of PAN-based carbon fiber precursors by small angle X-ray scattering. Chinese Physics C, 2011, 35(9): 870-874. doi: 10.1088/1674-1137/35/9/016
[10]	WANG Huan-Hua . In-situ monitoring of EuTiO₃ and SrTiO₃ film growth using time-resolved X-ray scattering during pulsed-laser deposition. Chinese Physics C, 2009, 33(11): 935-943. doi: 10.1088/1674-1137/33/11/002
[11]	YAN Li-Xin , DU Ying-Chao , DU Qiang , LI Ren-Kai , HUA Jian-Fei , HUANG Wen-Hui , TANG Chuan-Xiang . TW Laser system for Thomson scattering X-ray light source at Tsinghua University. Chinese Physics C, 2009, 33(S2): 154-156. doi: 10.1088/1674-1137/33/S2/040
[12]	TANG Chuan-Xiang , LI Ren-Kai , HUANG Wen-Hui , CHEN Huai-Bi , DU Ying-Chao , DU Qiang , DU Tai-Bin , HE Xiao-Zhong , HUA Jian-Fei , LIN Yu-Zhen , QIAN Hou-Jun , SHI Jia-Ru , XIANG Dao , YAN Li-Xin , YU Pei-Cheng . A simulation study of Tsinghua Thomson scattering X-ray source. Chinese Physics C, 2009, 33(S2): 146-150. doi: 10.1088/1674-1137/33/S2/038
[13]	SHI Jia-Ru , CHEN Huai-Bi , TANG Chuan-Xiang , HUANG Wen-Hui , DU Ying-Chao , ZHENG Shu-Xin , REN Li . RF deflecting cavity for bunch length measurement in Tsinghua Thomson scattering X-ray source. Chinese Physics C, 2009, 33(S2): 161-164. doi: 10.1088/1674-1137/33/S2/042
[14]	LAN Ding , WANG Yu-Ren , ZHANG Yin-Min , WANG Wei , WU Zhong-Hua . Small angle X-ray scattering of the colloidal crystal. Chinese Physics C, 2009, 33(11): 1016-1018. doi: 10.1088/1674-1137/33/11/017
[15]	YE Yun-Xiu , YAN Xin-Hu , CHENG He-Ping , JIAO Zheng , HE Ju , LIU Ren-Chen , . Nitrogen dilution effect for small angle GDH experiment in JLab Hall-A. Chinese Physics C, 2008, 32(8): 653-656. doi: 013
[16]	HONG Xin-Guo , WENG Yu-Xiang , LI Ming . Low Resolution Structure of Integral Membrane Protein Light-harvesting Complex LH2 From Photosynthetic Bacteria in the Detergent Solution by Synchrotron Small-angle X-ray Scattering. Chinese Physics C, 2003, 27(S1): 57-62.
[17]	ZHAO Hui , WU Zhong-Hua , GUO Mei-Fang , DONG Bao-Zhong . Evaluation of Structural Parameters in Semicrystalline Polymer Three-Phase Systems by Small-Angle X-Ray Scattering. Chinese Physics C, 2003, 27(11): 1034-1038.
[18]	ZHAO Hui , LI Zhi-Hong , DONG Bao-Zhong , RONG Li-Xia , WANG Jun . Preliminary Transformation on Experimental Data of Small Angle X-Ray Scattering. Chinese Physics C, 2001, 25(S1): 81-84.
[19]	Wang Jun , Cui Mingqi , Xu Wenxuan , Huang Yuying , Wang Dewu , Liu Jianfei , Xian Dingchang . Study of Multilayer Structures by Synchrotron Radiation with Small Angle Diffraction Method. Chinese Physics C, 1993, 17(S4): 311-319.
[20]	WAN Dai-Rong , CAO Jian-Hua , DAI Yun-Sheng , LIANG Xue-Cai . Measurement of Small-Angle Scattering of 14.7MeV Neutrons by C and Be Nuclei. Chinese Physics C, 1991, 15(2): 165-169.

Access

Get Citation

LIU Guan-Fen, WANG We-Jia, XU Jia-Hua and DONG Yu-Hui. Small-angle X-ray scattering probe of intermolecularinteraction in red blood cells[J]. Chinese Physics C, 2015, 39(3): 038001. doi: 10.1088/1674-1137/39/3/038001

RIS(for EndNote,Reference Manager,ProCite)

BibTex

Txt

Milestone

Received: 2014-04-07
Revised: 2014-08-12

Article Metric

Article Views(1782)
PDF Downloads(137)
Cited by(0)

Policy on re-use

To reuse of subscription content published by CPC, the users need to request permission from CPC, unless the content was published under an Open Access license which automatically permits that type of reuse.

通讯作者: 陈斌, [email protected]

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Small-angle X-ray scattering probe of intermolecularinteraction in red blood cells

Corresponding author: DONG Yu-Hui,

Received Date: 2014-04-07

Accepted Date: 2014-08-12

Available Online: 2015-03-05

Abstract: With high concentrations of hemoglobin (Hb) in red blood cells, self-interactions among these molecules could increase the propensities of their polymerization and aggregation. In the present work, high concentration Hb in solution and red blood cells were analyzed by small-angle X-ray scattering. Calculation of the effective structure factor indicates that the interaction of Hb molecules is the same when they are crowded together in both the cell and physiological saline. The Hb molecules stay individual without the formation of aggregates and clusters in cells.

Small-angle X-ray scattering probe of intermolecularinteraction in red blood cells

Abstract：

Access

Article Metrics

Metrics

通讯作者: 陈斌, [email protected]

Email This Article

Small-angle X-ray scattering probe of intermolecularinteraction in red blood cells

Corresponding author: DONG Yu-Hui,

HTML

目录